Each barcode adapter also has a cohesive end and this is used as a hook to ligate to the supplied sequencing adapters. Barcoded samples are then pooled together. The library preparation method is similar to the Ligation Sequencing Kit protocol DNA ends are repaired and dA-tailed using the NEBNext End Repair/dA-tailing module, and then a unique dT-tailed barcode adapter is ligated on the dA-tailed template. cDNA – use in combination with Direct cDNA Sequencing Kit.gDNA – use in combination with Ligation Sequencing Kit.The Native Barcoding Expansion 1-12 is an expansion pack, which provides 12 unique barcodes to enable PCR-free multiplexing of dsDNA samples such as: Multiplexing samples onto one flow cell can reduce the cost per sample for a user. If customers require further support for any ongoing critical experiments using a Legacy product, please contact Customer Support via email: or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow-cell. This kit is soon to be discontinued and we recommend all customers to upgrade to the latest chemistry for their relevant kit which is available on the Store. Please note: This is only compatible with Kit 9 chemistry. Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail. are interested in utilising upstream processes such as size selection or whole genome amplification.want to optimise their sequencing experiment for throughput. need a PCR-free method of multiplexing to preserve additional information such as base modifications.wish to multiplex samples to reduce price per sample.
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